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| Pereira,Marlon Corrêa; Rocha,Diego Ismael; Veloso,Tomás Gomes Reis; Pereira,Olinto Liparini; Francino,Dayana Maria Teodoro; Meira,Renata Maria Strozi Alves; Kasuya,Maria Catarina Megumi. |
| Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp.) promoted protocorm development until... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ceratorhiza; Epulorhiza; Orchid conservation; Seedling production; Symbiosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567 |
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| Carvalho,Otieres Cirino de; Paiva Neto,Vespasiano Borges de; Padilha,Daly Roxana Castro; Veloso,Tomás Gomes Reis; Bocayuva,Melissa Faust; Soares,Deanna Carla Oliveira; Kasuya,Maria Catarina Megumi. |
| ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; ITS-DNAr; Mycorrhiza; Orchidaceae; Symbiosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100107 |
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| Guimarães,Fernanda Aparecida Rodrigues; Pereira,Marlon Corrêa; Felício,Cristiane da Silva; Torres,Denise Pereira; Oliveira,Sabrina Feliciano; Veloso,Tomás Gomes Reis; Kasuya,Maria Catarina Megumi. |
| In nature, orchid seeds obtain the nutrients necessary for germination by degrading intracellular fungal structures formed after colonization of the embryo by mycorrhizal fungi. Protocols for asymbiotic germination of orchid seeds typically use media with high concentrations of soluble carbohydrate and minerals. However, when reintroduced into the field, seedlings obtained via asymbiotic germination have lower survival rates than do seedlings obtained via symbiotic germination. Tree fern fiber, the ideal substrate for orchid seedling acclimatization, is increasingly scarce. Here, we evaluated seed germination and protocorm development of Cyrtopodium glutiniferum Raddi cultivated in asymbiotic media (Knudson C and Murashige & Skoog) and in oatmeal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epulorhiza sp.; Symbiosis; Orchid; Substrate; Fern fiber. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000300016 |
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